The depot served as a hub for transportation in the area, connecting Dade City with other parts of Florida and the rest of the country. The railroad brought passengers, mail, and goods into town and helped boost the local economy.
During World War II, the depot was a busy center of activity as soldiers and supplies passed through on their way to training camps and military bases. After the war, the depot continued to serve the community until passenger service was discontinued in the 1960s.
In the 1980s, the depot was restored and converted into a museum. Today, visitors can tour the depot and see exhibits on the town's past, including the agriculture, cattle ranching, and citrus industries that once thrived in the area. The Dade City Train Depot is listed on the National Register of Historic Places.